Small Activation RNA Generator
Small Activation RNA Generator
Designing efficient small activating RNAs (saRNA) requires precision. This tool streamlines the process by converting your target DNA sequences into functional Sense and Guide (Antisense) RNA strands instantly. Version 7.6 introduces a critical GC Content Analysis feature, automatically flagging sequences that fall outside the optimal thermodynamic range (35-55%), ensuring your designs have the highest probability of successful gene activation.
Key Features:
Automated Strand Generation: Instantly generates Sense and Antisense (Guide) strands from any raw DNA input.
GC Content Optimization: Real-time calculation of GC percentages. The system visually flags candidates as "Optimal" (Green) or "Risk" (Red) based on the 35-55% stability rule.
Duplex Visualization: View the hybridization of the sense and antisense strands in a classic 5'-3' alignment.
Batch Comparison: Select multiple candidates to generate a side-by-side comparison table for easy export to your lab notebook.
Flexible Window Size: Default is 21 nucleotides, but fully adjustable for specific experimental needs.
How to Use:
Paste Sequence: Enter your target promoter DNA sequence into the input box (auto-cleaning included).
Set Parameters: Adjust the window size if needed (Default: 21nt).
Generate: Click "Generate & Select" to process the sequence.
Analyze & Select: Review the generated cards. Check the GC badges for stability warnings. Click "Select" on the best candidates.
Compare & Export: Use the bottom dock to open the "Comparison Table" and copy your final designs to the clipboard.