Small Activation RNA Designer 

This tool is an in-silico bioinformatics tool developed for designing Small Activating RNA (saRNA) sequences to increase the expression of target genes using the RNA Activation (RNAa) mechanism. The system scans the promoter region of the target gene and filters the most suitable candidates based on the following scientific criteria:

• Thermodynamic Stability: The Gibbs Free Energy (Δ G ) of each candidate is calculated using the Nearest-Neighbor method.

• Asymmetry Rule: The thermodynamic instability (A/T abundance) at the 3' end of the sense strand is analyzed to ensure proper loading onto the Argonaute 2 (Ago2) protein.

• Sequence Features: Positional preferences are scored based on the GC ratio (35-65%), homopolymer repeats, and Reynolds/Ui-Tei rules.

!!! This tool is designed for pre-labor pre-screening purposes, and in vitro validation of the results is recommended.