small actıvatıon rna antısense-sense Converter
small actıvatıon rna antısense-sense Converter
This tool is designed for precision engineering of small activating RNAs. Unlike batch generators, this converter focuses on validating specific target sequences. Simply input your DNA target, and the tool will generate the corresponding Sense (Passenger) and Antisense (Guide) RNA strands, visualize the duplex alignment, and calculate the GC content percentage to ensure thermodynamic stability.
Key Features:
Instant DNA-to-RNA Conversion: Automatically converts raw DNA inputs into Sense and Antisense RNA sequences (T -> U conversion included).
Smart GC Logic: Calculates the Guanine-Cytosine content in real-time. It provides an immediate "Optimal" (35-55%), "Low", or "High" status badge to help you assess experimental viability.
Duplex Visualization: distinct visual representation of the 5'-3' vs 3'-5' strand alignment to verify binding compatibility.
Order-Ready Output: Specifically extracts the Guide Strand in the standard 5'->3' direction required for chemical synthesis ordering.
How to Use:
Input Sequence: Paste your specific DNA target sequence (Promoter region) into the input field.
Generate: Click the "Generate Duplex & Analyze" button.
Review Stability: Check the GC Content stats panel. A green badge indicates an optimal range for gene activation.
Get Sequences: Use the "Copy Sequences" button to save the Sense and Guide strands to your clipboard.