References & Scıentıfıc Basıs 

Scientific References

The algorithms utilized in Dual-Target RNA Designer are built upon established bioinformatic frameworks:

1. Thermodynamics: Xia, T., et al. (1998). Biochemistry, 37(42). (Basis for Δ G calculations).

2. siRNA Design: Reynolds, A., et al. (2004). Nature Biotechnology, 22(3) & Ui-Tei, K., et al. (2004). Nucleic Acids Research, 32(3). (Basis for scoring and filtering).

3. RNA Activation (RNAa): Li, L.C., et al. (2006). PNAS, 103(46). (Mechanism for transcriptional upregulation).